Survival and recovery of asparagus shoot tips after cryopreservation using the “droplet” method
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چکیده
منابع مشابه
Cryopreservation of Potato Shoot Tips
Potato is one of the most important staple crops in both developed and developing countries of the world. Potato genetic resources are a prerequisite for potato breeding in both conventional and genetic engineering programmes. Cryopreservation has long been considered an ideal means for the long-term conservation of plant genetic resources. This review provides comprehensive information on vari...
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The aim of the present study was to investigate the importance of the preculture conditions on the survival of apple (Malus domestica Borkh., cv. Romus4 and rootstock M106) shoot tips cryopreserved using a combined droplet vitrification procedure. Preculture was critical for the survival of apple shoot tips after cryopreservation. Among the different tested sugars and sugar alcohols (glucose, s...
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Vitrification refers to the physical process by which a liquid supercools to very low temperatures and finally solidifies into a metastable glass, without undergoing crystallization at a practical cooling rate. Thus, vitrification is an effective freeze-avoidance mechanism and living tissue cryopreservation is, in most cases, relying on it. As a glass is exceedingly viscous and stops all chemic...
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Cryopreservation of in vitro-grown shoot tips of cassava by encapsulation-vitrification method.
Shoot tips of cassava (Manihot esculenta Crantz) in vitro plantlets were successfully cryopreserved using the encapsulation-vitrification technique. Nodal cuttings of 5 mm length with one leaf were cultured on modified MS medium in Petri dishes (90 mm x 20 mm) for about 28 days. Excised shoot tips were precultured on sucrose enriched (0.3 M) medium for 16 h, encapsulated and osmoprotected with ...
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ژورنال
عنوان ژورنال: New Zealand Journal of Crop and Horticultural Science
سال: 2000
ISSN: 0114-0671,1175-8783
DOI: 10.1080/01140671.1997.9514150